The heir-less Zhengde's death in April 1521 brought upon disarray in the imperial court. The Templar eunuchs Eight Tigers used this opportunity for plotting to control China by controlling the most influential people in court. They placed Zhengde's cousin Jiajing in the throne and ruled China through his stead.[1]

In 1524, the Eight Tigers triggered the Great Rites Controversy to hunt down officials who tried to oppose the Jiajing Emperor, specifically purging the Chinese Assassins. They managed to chase and kill the Mentor Zhu Jiuyuan, who was in Italy seeking help with the now Assassin Shao Jun and eradicate most of the Brotherhood.[1]

After receiving training with the legendary Master Assassin Ezio Auditore da Firenze,[2] Shao Jun returned to China in 1526 to exact vengeance with her old Mentor Wang Yangming. By using a Precursor box, they assassinated the majority of the Eight Tigers until Zhang Yong retrieved the box, after killing Wang Yangming.[1]

By 1532, Jun pursued Zhang Yong, the last Tiger, who was planning to let the Altan Khan and his Mongols invade China in the Great Wall. The Assassin killed the Mongol scouts and closed the Wall's gates, preventing the invaders to breach her home. Among the chaos of bombardment from the Mongols, Shao Jun managed to kill Zhang Yong, eliminating the Templar threat in China.[1]

From 1542 to 1550, China was continually harassed by the Mongols. Jiajing eventually ended the conflict by offering the Mongols special trading rights and expanded Peking by building the Outer City. Jiajing developed an interest in alchemy in his later years which led to his death after the Assassins played on his desire to drink the elixir of life and arranged for him to ingest a lethal dose of mercury.[1]