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巴比伦刺客兄弟会（Babylonian Brotherhood of Assassins）是刺客组织位于巴比伦尼亚的支派。
Although Babylonia had by then ceased to be a sovereign entity, its Assassin branch was still active when western Asia fell to the Macedonian Empire of Alexander the Great. One of its agents, Iltani, set out to assassinate Alexander during his campaign in what is now modern-day Afghanistan, but she was forced to withdraw when she found him too well-guarded in the citadel of Herat. As a result, she turned to an alchemist of the former Achaemenid Empire who provided her with a deadly but slow-acting poison. 在公元前323年6月13日左右，巴比伦刺客伊尔塔尼向接受了圣殿骑士帮助并受委托保管伊甸权杖的马其顿国王亚历山大大帝下毒。