中国刺客兄弟会（Chinese Brotherhood of Assassins）是指那些在中国活动的刺客兄弟会成员。他们是少数存在了千年以上的兄弟会之一。
The conflict dragged on for the next few decades with the Song's grasp on the country continuing to crumble before the might of the Mongol forces. In 1259, a Chinese Assassin perished in the midst of saving Diaoyu Fortress from a Mongol assault, leaving his daughter, Zhang Zhi, to discover his corpse in the aftermath. Despite his doubts about her motives, Kang, the late Assassin's Mentor, acquiesced to adopting Zhi into the order and completing her training. His misgivings were proven true when Zhi defied his warnings to prioritize duty over revenge and personally infiltrated the Mongol camp to assassinate the Great Khan Möngke Khan. Though she was successful, she was crippled during her escape by the future Templar Bayan, and Kang used her disability as an added pretext for expelling her from the order.
1524年，新皇帝嘉靖再次受到圣殿骑士的帮助下，几乎摧毁了中国的刺客组织，determined to eliminate all groups who opposed him。This determination led him to execute all of Zhengde's former concubines, their death by 凌遲 discovered by Shao Jun and her Mentor on the Assassins' second rescue attempt.
Shao Jun's quest was a success; she managed to kill the Eight Tigers, including their Master, Zhang Yong, in 1532. Her Mentor, Wang Yangming, was killed by Yong three years prior. They also succeeded in assassinating Emperor Jiajing by giving him a false elixir of life. By 1582, her Brotherhood was in contact with their Japanese brothers, with Liu Yan being present in the country with the task of taking Oda Nobunaga's Sword of Eden to safety, following Nobunaga's assassination by Yamauchi Taka.
By 2015, at least one Assassin cell operated in China. That year, they were aided by the Japanese Assassins, including Kiyoshi Takakura, who were sent by Japanese Mentor Saeko Mochizuki, in their search for Álvaro Gramática's secret laboratory in which he planned to replicate an Isu's body with the help of the Shroud.
In 2016, the Chinese Assassin Yanmei was ordered by Assassin leader Gavin Banks to assist another Assassin, Griffin, and the teenagers Natalya Aliyev, David Collins and Javier Mondragón in finding the Tomb of Möngke Khan and retrieving a prong of the Trident of Eden. However, Yanmei was ultimately killed by the Templar Isaiah after he recovered the fear prong and used its power against everyone, killing her when she tried to attack him.
In February 2017, an Assassin cell led by Barindra Mitra assisted Charlotte de la Cruz in her efforts to gather informations about the Templars' Phoenix Project. Infiltrating an office of Abstergo in a skyscraper in Hong Kong, which had in fact been abandoned by the Templars, the Assassins were ambushed by an unidentified group. Barindra and her cell were all killed with only de la Cruz and the tech expert, Guernica Moneo, escaping.